Journal: Biology of reproduction

Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.

doi: 10.1095/biolreprod.106.059410

Figure Lengend Snippet: FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

Article Snippet: Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.

Techniques: Western Blot, Control, Positive Control, Transformation Assay

Journal: Frontiers in Immunology

Article Title: Fibrocytes: A Critical Review and Practical Guide

doi: 10.3389/fimmu.2021.784401

Figure Lengend Snippet: Anti-collagen antibodies.

Article Snippet: FC , Mouse Heart Cells , IF for pCol1a1 , Biotinylated-Anti-Collagen I A , Rockland Immunochemicals , 600-401-103.

Techniques: Biomarker Discovery, Expressing, Cell Culture, In Situ Hybridization, Western Blot

Journal: The Journal of physiology

Article Title: Metabolic remodelling of glucose, fatty acid and redox pathways in the heart of type 2 diabetic mice

doi: 10.1113/JP276824

Figure Lengend Snippet: Depicted is a scheme of the model of central catabolism of oxidative pathways from glucose and FAs (Palm) in cytoplasmic and mitochondrial compartments. Open arrows indicate the exchange of the metabolite with the extracellular medium. Filled arrows indicate intracellular enzymes/processes. Enzymes: (1) HK, hexokinase; (2) PFK, phosphofructokinase; (3) ALD, aldolase; (4) GAPD, glyceraldehyde 3 phosphate dehydrogenase; (5) PGK, phosphoglycerol kinase; (6) Eno, enolase; (7) PK, pyruvate kinase; (8) G6PD, glucose 6 phosphate dehydrogenase; (9) 6 PGO, 6 phosphogluconate dehydrogenase; (10) R5PI, ribose 5P isomerase; (11) Ru5PE, ribulose 5P epimerase; (12) TK1, transketolase 1; (13) TAL, transaldolase; (14) TK2, transketolase 2; (15) XDH, xylitol dehydrogenase; (16) Gno_is, glycogen breakdown; (17) GPa,b, glycogen phosphorylase; (18) PGLM, phosphoglucomutase; (19) ALDR, aldose reductase; (20) SoDH, sorbitol dehydrogenase; (21) LDH, lactate dehydrogenase; (22) PyrT, pyruvate carrier; (23) PDH, pyruvate dehydrogenase; (24) CPT1, carnitine palmitoyl transferase 1; (25) CAD, fatty acyl CoA dehydrogenase; (26) bMTP, beta-oxidation multienzyme complex; (27) PyrCb, pyruvate carboxylase; (28) ME, malic enzyme; (29) CS, citrate synthase; (30) Aco, aconitase; (31) IDH, isocitrate dehydrogenase; (32) aKGDH, alpha-ketoglutarate dehydrogenase; (33) SL, succinyl CoA lyase; (34) SDH, succinate dehydrogenase; (35) FH, fumarase; (36) MDH, malate dehydrogenase; (37) AAT, aspartate amino transferase. Metabolites: 1,3BPG, 1,3-bisphosphoglycerate; 3PG, 3-phosphoglycerate; 6PGluc, 6-phosphogluconate; AcCoA, acetyl-CoA; C16CoA, palmitoyl-CoA; Cit, citrate; F1,6BP, fructose 1,6-bisphosphate; G1P, glucose 1-phosphate; G3P, glyceraldehyde 3-phosphate; hexose-6P, accounts for glucose 6-phosphate plus fructose 6-phosphate; Lac, lactate; (Maltose)n, maltooligosaccharides (maltose, malto-triose, -tetraose, -hexaose, etc.); OAA, oxaloacetate; PEP, phosphoenolpyruvate; Pyr, pyruvate; R5P, ribose 5-phosphate; Ru5P, ribulose 5-phosphate; Succ, succinate; X5P, xylulose 5-phosphate.

Article Snippet: With lipid, the ratio of Palm oxidation over glucose uptake shifted from 0.024 to 0.026 in WT and from 0.032 to 0.0525 in T2DM, indicating that in the db / db heart the presence of lipid increases 64% its relative oxidation with respect to glucose. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 12. caption a7 caption a8 Fluxome remodelling and flux distribution in central catabolism from WT and diabetic mouse hearts Depicted are the fluxes (in μ M s −1 ) sustained by the pathways involved in glucose and FA utilization.

Techniques:

Journal: The Journal of physiology

Article Title: Metabolic remodelling of glucose, fatty acid and redox pathways in the heart of type 2 diabetic mice

doi: 10.1113/JP276824

Figure Lengend Snippet: Depicted are the fluxes (in μM s−1) sustained by the pathways involved in glucose and FA utilization. Metabolite concentrations (in mM) were determined as described in Methods and used as inputs for the calculation of fluxes (in μM s−1, equivalent to nmol s−1 mL−1 intracellular water) sustained by the pathways involved in glucose and FA utilization in cytoplasmic and mitochondrial compartments (see legend to Fig. 1). For flux calculation we used our computational model of central catabolism, which was validated by its ability to reproduce the metabolite concentrations determined experimentally (Tables 3 and ​and4),4), after metabolite profiling of WT and T2DM hearts perfused under the conditions described in the legend of Fig. 2. Boxed are the flux values corresponding to WT and diabetic hearts (left and right, underlined, number columns, respectively) next to their respective steps in the network. Within each box, the experimental condition G, GI or GIP is denoted by a distinct colour (red, black, blue, respectively). For abbreviations, see legend to Fig. 1.

Article Snippet: With lipid, the ratio of Palm oxidation over glucose uptake shifted from 0.024 to 0.026 in WT and from 0.032 to 0.0525 in T2DM, indicating that in the db / db heart the presence of lipid increases 64% its relative oxidation with respect to glucose. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 12. caption a7 caption a8 Fluxome remodelling and flux distribution in central catabolism from WT and diabetic mouse hearts Depicted are the fluxes (in μ M s −1 ) sustained by the pathways involved in glucose and FA utilization.

Techniques:

Journal: The Journal of physiology

Article Title: Metabolic remodelling of glucose, fatty acid and redox pathways in the heart of type 2 diabetic mice

doi: 10.1113/JP276824

Figure Lengend Snippet: The fluxome of diabetic (right, red) and WT (left, blue) are depicted, highlighting major differences in the fluxes as denoted by arrow thickness. Fluxes throughout central catabolism were about 2-fold lower in glucose metabolism from diabetic heart. Also displayed is the higher flux through the ‘lower’ part of the TCA cycle in the T2DM heart, in contrast with the WT where the ‘upper’ part predominates. For abbreviations, see legend to Fig. 1.

Article Snippet: With lipid, the ratio of Palm oxidation over glucose uptake shifted from 0.024 to 0.026 in WT and from 0.032 to 0.0525 in T2DM, indicating that in the db / db heart the presence of lipid increases 64% its relative oxidation with respect to glucose. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 12. caption a7 caption a8 Fluxome remodelling and flux distribution in central catabolism from WT and diabetic mouse hearts Depicted are the fluxes (in μ M s −1 ) sustained by the pathways involved in glucose and FA utilization.

Techniques: